New stool test codes

The traditional culture method has been replaced by Real Time PCR for enteric pathogen testing. The benefits are increased sensitivity and a higher detection rate. Once received and processed in the microbiology lab, negative results will be available within 24 hours. Positive results will be followed up with culture and sensitivities for final reporting.

 

Stool OCP and culture

Sample type

Current test & method (SPAR)

New method – change of
Test Code from SPAR to PENT.
Please request as PENT**

Comments

Stool

Stool for Ova, Cysts & Parasites (OCP) Microscopy and Culture

Serosep EntericBio PCR

Bacteria/Bacterial Toxins

  • Salmonella
  • Campylobacter
  • Shigella
  • VTEC

Parasites

  • Cryptosporidium
  • Giardia

All stool samples will be tested for UK Pathogens.

Overseas pathogens will only be tested if requested and travel history and clinical details are provided. Samples that are positive for the bacterial pathogens will be cultured to provide sensitivities and, if indicated, for PHE referral.

Samples will be kept for 7 days after receipt to allow for additional testing if required.


**Please add the code PENT to your practice system’s test menu if you request electronically.

 

Stool for OCP

Sample type

Current test & method (OCP)

New method – Test code remains unchanged: OCP

Comments

Stool

Stool for Ova, Cysts & Parasites (OCP)

Requests for OCP only will include testing for cryptosporidium and giardia by PCR.

Overseas pathogens will only be tested if requested and travel history and clinical details are provided.

 

C. Difficile Detection

Sample type

Current test & method (CLOS)

New method – Test code remains unchanged: CLOS

Comments

Stool

Alere Techlab Duo Card (GDH & Toxin combo)

Serosep Enteric Bio PCR (GDH)

Alere Techlab EIA (Toxin)

Change to PCR and Elisa methods.

Two tier GDH & Toxin c. diff screening based on PHE guidance. Improved sensitivity and specificity for both targets tested.

Primary c. diff gene screening using Enteric Bio PCR.

Secondary sequential testing using Alere EIA to confirm Toxin.

 

Gastro Virus Detection (including Rotavirus)

Sample type

Current test & method (ROTA)

New method – Test code remains unchanged: ROTA

Comments

Stool

Launch Meridian Rotavirus Immunocard

Bioconnection Rotavirus Immunocard

Multiplex Gastro PCR

  • Rotavirus
  • Pan Adenovirus
  • Adenovirus Type F

Improved sensitivity and specificity for combined rotavirus and adenovirus targets using PCR.

Change to PCR method for improved sensitivity and specificity.

Additional PCR panel for Norovirus 1&2 is also available (request as NORO).

 

Enteric Organism Rapid Detection

Sample type

Current test & method (EORD)

No change – Test Code remains unchanged: EORD

Comments

Stool

xTAG® Gastrointestinal Pathogen Panel Luminex 200

Multiplex PCR

Simultaneous Detection of 15 nucleic acids from multiple gastroenteritis causing Viruses, Parasites and Bacteria.

This profile includes the following:

Bacteria and Bacterial Toxins: Salmonella, Shigella, Campylobacter, Clostridiium difficile Toxin A/B, Enterotoxigenic E. Coli (ETEC) LT/ST, E. coli 0157, Shiga-like Toxin Producing E. coli (STEC) stx 1/stx 2, Vibrio cholera, Yersinia entericolitica

Viruses: Adenovirus 40/41, Rotavirus A, Norvirus G1/G11

Parasites: Gardia, Entamoeba histolytica, Cryptosporidium